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Anti-IGF2BP3 Antibody
Catalog #: 144-04444
IGF2BP3 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | O00425 |
| Gene Id | 10643 |
| Gene Symbols | IGF2BP3 |
| Protein Name / Synonyms | IGF2BP3, CT98, IMP-3, IMP3, KOC, KOC1, VICKZ3, insulin-like growth factor 2 mRNA-binding protein 3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF, IP |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene is primarily found in the nucleolus, where it can bind to the 5' UTR of the insulin-like growth factor II leader 3 mRNA and may repress translation of insulin-like growth factor II during late development. The encoded protein contains several KH domains, which are important in RNA binding and are known to be involved in RNA synthesis and metabolism. A pseudogene exists on chromosome 7, and there are putative pseudogenes on other chromosomes.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:20 - 1:50
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:20 - 1:50
Format
Calculated MW
21kDa/63kDa
Observed MW
70kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 300-579 of human IGF2BP3 (NP_006538.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using IGF2BP3 antibody (144-04444) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.
Immunohistochemistry of paraffin-embedded human esophagus using IGF2BP3 antibody (144-04444) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using IGF2BP3 antibody (144-04444) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using IGF2BP3 antibody (144-04444) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of U2OS cells using IGF2BP3 antibody (144-04444) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 100ug extracts of HepG2 cells using 3ug IGF2BP3 antibody (144-04444). Western blot was performed from the immunoprecipitate using IGF2BP3 antibody (144-04444) at a dilition of 1:1000.
Expiration:
12 months from the date of shipment when stored properly.