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Anti-IGF2R Antibody
Catalog #: 144-60151
IGF2R Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P11717 |
| Gene Id | 3482 |
| Gene Symbols | IGF2R |
| Protein Name / Synonyms | IGF2R, CD222, CI-M6PR, CIMPR, M6P-R, M6P/IGF2R, MPR 300, MPR1, MPR300, MPRI, insulin like growth factor 2 receptor |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Concentration (lot specific) | 3.28 mg/ml |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a receptor for both insulin-like growth factor 2 and mannose 6-phosphate. The binding sites for each ligand are located on different segments of the protein. This receptor has various functions, including in the intracellular trafficking of lysosomal enzymes, the activation of transforming growth factor beta, and the degradation of insulin-like growth factor 2. Mutation or loss of heterozygosity of this gene has been association with risk of hepatocellular carcinoma. The orthologous mouse gene is imprinted and shows exclusive expression from the maternal allele; however, imprinting of the human gene may be polymorphic, as only a minority of individuals showed biased expression from the maternal allele (PMID:8267611).
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
274kDa
Observed MW
310kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 2327-2491 of human IGF2R (NP_000867.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of HeLa cells, using IGF2R antibody (144-60151) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human placenta using IGF2R antibody (144-60151) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using IGF2R antibody (144-60151) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using IGF2R antibody (144-60151) at dilution of 1:100. Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.