Anti-IKBKG Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	Q9Y6K9
Gene Id	8517
Gene Symbols	IKBKG
Protein Name / Synonyms	IKBKG, AMCBX1, FIP-3, FIP3, Fip3p, IKK-gamma, IKKAP1, IKKG, IMD33, IP, IP1, IP2, IPD2, NEMO, ZC2HC9, NF-kappa-B essential modulator
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IP
Research Area	Cardiovascular Disease, Inflammation, Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes the regulatory subunit of the inhibitor of kappaB kinase (IKK) complex, which activates NF-kappaB resulting in activation of genes involved in inflammation, immunity, cell survival, and other pathways. Mutations in this gene result in incontinentia pigmenti, hypohidrotic ectodermal dysplasia, and several other types of immunodeficiencies. A pseudogene highly similar to this locus is located in an adjacent region of the X chromosome.

Dilutions

WB 1:500 - 1:2000
IP 1:50 - 1:100

Format

Calculated MW

36kDa/48kDa/55kDa

Observed MW

48kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-419 of human IKBKG (NP_003630.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using IKBKG Antibody (144-12536) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - [KO Validated] IKBKG Polyclonal Antibody (144-12536)

Western blot analysis of extracts from normal (control) and IKBKG knockout (KO) 293T cells, using IKBKG antibody (144-12536) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Western blot - [KO Validated] IKBKG Polyclonal Antibody (144-12536)

Immunoprecipitation analysis of 200ug extracts of A549 cells using 3ug IKBKG antibody (144-12536). Western blot was performed from the immunoprecipitate using IKBKG antibody (144-12536) at a dilition of 1:1000.
Immunoprecipitation - [KO Validated] IKBKG Polyclonal Antibody (144-12536)

Expiration:

12 months from the date of shipment when stored properly.

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