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Anti-IL10 Antibody

Catalog #: 130-10230

Mouse anti-IL10 (5E8-C9-F6) Antibody

Product Description

Specifications

Size20 µg, 100 µg, 200 µg, 500 µg
Estimated Lead Time1-2 business days
SpeciesHuman
Specificity
This antibody binds to its immunogen and did not show any cross reactivity with unrelated antigens in ELISA. Cross reactivity with mouse and rat IL-10 has not been tested yet.
Accession Number
NP_000563
ClonalityMonoclonal
Immunogen
Immunogen was recombinant protein. This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with the immunogen. The IgG fraction of tissue culture supernatant was purified by Protein G/A affinity chromatography.
Recommended ApplicationsELISA (recommended work dilution= 1:640, 000), Western Blotting (recommended work dilution= 1:1, 000-5, 000),
Antigen Catalog Number
230-00242
Shipping TypeBlue ice
Storage-20°C

Description

Description

Interleukin-10 (IL-10), also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. In humans, IL-10 is encoded by the IL10 gene.

Immunohistochemistry (IHC) Analysis

IHC showed positive staining in human colon tumor tissue. The mouse Anti-Human IL-10 antibody (130-10230) was used at a 200-fold dilution, followed by mouse and rabbit IgG (ready to use) polymerization using HRP polymer, and counterstained with hematoxylin. Heat-mediated antigen retrieval was performed using Tris/EDTA buffer at pH9.0 before beginning the IHC staining (40X).

Storage/Stability

Store at 4°C if intended for use within one month, otherwise, store at -20°C to -80°C. The lyophilized antibody is stable for at least 18 months after the date of receipt when stored at -20°C to -80°C. After reconstitution, it can be aliquotted and stored at 4°C for 1 month or at -20°C to -80°C in a manual defrost freezer for many months without detectable loss activity.

Expiration

12 months from the date of shipment when stored properly.