Anti-IL12A (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P29459
Gene Id	3592
Gene Symbols	IL12A
Protein Name / Synonyms	IL12A (C-term)
Clonality	Polyclonal
Preparation	This IL12A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 168-195 amino acids from the C-terminal region of human IL12A.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC
Research Area	Angiogenesis, Cancer
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

IL12A is a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity.

Specificity

Other Names

IL12A; NKSF1; Interleukin-12 subunit alpha; Cytotoxic lymphocyte maturation factor 35 kDa subunit; IL-12 subunit p35; NK cell stimulatory factor chain 1

NCBI Accession #

NP_000873.2

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

24874

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50; FC: 1:10-50

	Western blot analysis of IL12A Antibody (C-term) (Cat# 102-18033) in MDA-MB231 cell line lysates (35ug/lane). IL12A (arrow) was detected using the purified Pab.
	Formalin-fixed and paraffin-embedded human breast carcinoma reacted with IL12A Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	IL12A Antibody (C-term) (Cat# 102-18033) flow cytometry analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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