3607 Parkway Ln Ste 200 Norcross, Georgia 30092
www.RayBiotech.com | info@raybiotech.com
770-729-2992 | +1-888-494-8555
ISO 13485:2016 cGMP
Anti-IL12RB1 Antibody
Catalog #: 144-01886
IL12RB1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P42701 |
| Gene Id | 3594 |
| Gene Symbols | IL12RB1 |
| Protein Name / Synonyms | IL12RB1, CD212, IL-12R-BETA1, IL12RB, IMD30, interleukin-12 receptor subunit beta-1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene is a type I transmembrane protein that belongs to the hemopoietin receptor superfamily. This protein binds to interleukine 12 (IL12) with a low affinity, and is thought to be a part of IL12 receptor complex. This protein forms a disulfide-linked oligomer, which is required for its IL12 binding activity. The coexpression of this and IL12RB2 proteins was shown to lead to the formation of high-affinity IL12 binding sites and reconstitution of IL12 dependent signaling. Mutations in this gene impair the development of interleukin-17-producing T lymphocytes and result in increased susceptibility to mycobacterial and Salmonella infections. Alternative splicing results in multiple transcript variants.
Dilutions
WB 1:500 - 1:2000
IF 1:10 - 1:100
IF 1:10 - 1:100
Format
Calculated MW
42kDa/72kDa/73kDa
Observed MW
73kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 25-305 of human IL12RB1 (NP_714912.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using IL12RB1 antibody (144-01886) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5min.
Immunofluorescence analysis of A549 cells using IL12RB1 antibody (144-01886). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.