Anti-IL21 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q9HBE4
Gene Id	59067
Gene Symbols	IL21
Protein Name / Synonyms	IL21, CVID11, IL-21, Za11, interleukin-21
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.09% Sodium azide, 50% glycerol, pH 7.3
Recommended Applications	Western Blotting, IHC
Research Area	Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a member of the common-gamma chain family of cytokines with immunoregulatory activity. The encoded protein plays a role in both the innate and adaptive immune responses by inducing the differentiation, proliferation and activity of multiple target cells including macrophages, natural killer cells, B cells and cytotoxic T cells. Dysregulation of this gene plays a role in multiple immune-mediated diseases including lupus, psoriasis and chronic inflammatory diseases. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200

Format

Calculated MW

16kDa/17kDa

Observed MW

18kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 25-162 of human IL21 (NP_068575.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using IL21 antibody (144-07235) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Western blot - IL21 antibody (144-07235)

Immunohistochemistry of paraffin-embedded rat spleen using IL21 antibody (144-07235) at dilution of 1:100 (40x lens).
Immunohistochemistry - IL21 antibody (144-07235)

Expiration:

12 months from the date of shipment when stored properly.

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