Anti-IL3RA Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P26951
Gene Id	3563
Gene Symbols	IL3RA
Protein Name / Synonyms	IL3RA, CD123, IL3R, IL3RAY, IL3RX, IL3RY, hIL-3Ra, interleukin-3 receptor subunit alpha
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene is an interleukin 3 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL3 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL3. This gene and the gene encoding the colony stimulating factor 2 receptor alpha chain (CSF2RA) form a cytokine receptor gene cluster in a X-Y pseudoautosomal region on chromosomes X or Y. Alternatively spliced transcript variants encoding distinct isoforms have been found.

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW

34kDa/43kDa

Observed MW

70kDa

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human IL3RA (NP_002174.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of A-549 cells, using IL3RA antibody (144-03926) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - IL3RA antibody (144-03926)

Expiration:

12 months from the date of shipment when stored properly.

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