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Anti-ILK Antibody
Catalog #: 144-00901
ILK Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q13418 |
| Gene Id | 3611 |
| Gene Symbols | ILK |
| Protein Name / Synonyms | ILK, HEL-S-28, ILK-1, ILK-2, P59, p59ILK, integrin linked kinase |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Research Area | Cancer |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a protein with a kinase-like domain and four ankyrin-like repeats. The encoded protein associates at the cell membrane with the cytoplasmic domain of beta integrins, where it regulates integrin-mediated signal transduction. Activity of this protein is important in the epithelial to mesenchymal transition, and over-expression of this gene is implicated in tumor growth and metastasis. Alternative splicing results in multiple transcript variants.
Dilutions
WB 1:500 - 1:1000
IHC 1:50 - 1:100
IHC 1:50 - 1:100
Format
Calculated MW
36kDa/44kDa/51kDa
Observed MW
48kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human ILK (NP_001014795.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using ILK antibody (144-00901) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded mouse kidney using ILK Antibody (144-00901) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using ILK Antibody (144-00901) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using ILK Antibody (144-00901) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using ILK Antibody (144-00901) at dilution of 1:200 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.