Anti-ING3 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q9NXR8
Gene Id	54556
Gene Symbols	ING3
Protein Name / Synonyms	ING3, Eaf4, ING2, MEAF4, p47ING3, inhibitor of growth protein 3
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF
Research Area	Cancer
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene is similar to ING1, a tumor suppressor protein that can interact with TP53, inhibit cell growth, and induce apoptosis. This protein contains a PHD-finger, which is a common motif in proteins involved in chromatin remodeling. This gene can activate p53 trans-activated promoters, including promoters of p21/waf1 and bax. Overexpression of this gene has been shown to inhibit cell growth and induce apoptosis. Allelic loss and reduced expression of this gene were detected in head and neck cancers. Two alternatively spliced transcript variants encoding different isoforms have been observed.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200

Format

Calculated MW

11kDa/46kDa

Observed MW

50kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-92 of human ING3 (NP_938008.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using ING3 antibody (144-05832) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Western blot - ING3 antibody (144-05832)

Immunofluorescence analysis of A549 cells using ING3 antibody (144-05832). Blue: DAPI for nuclear staining.
Immunofluorescence - ING3 antibody (144-05832)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products