Anti-ING5 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q8WYH8
Gene Id	84289
Gene Symbols	ING5
Protein Name / Synonyms	ING5, p28ING5, inhibitor of growth protein 5
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a tumor suppressor protein that inhibits cell growth and induces apoptosis. This protein contains a PHD-type zinc finger. It interacts with tumor suppressor p53 and p300, a component of the histone acetyl transferase complex, suggesting a role in transcriptional regulation. Alternative splicing and the use of multiple promoters and 3' ends results in multiple transcript variants.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:100

Format

Calculated MW

25kDa/27kDa

Observed MW

28kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human ING5 (NP_115705.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using ING5 antibody (144-07288) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Western blot - ING5 antibody (144-07288)

Immunofluorescence analysis of MCF-7 cells using ING5 antibody (144-07288). Blue: DAPI for nuclear staining.
Immunofluorescence - ING5 antibody (144-07288)

Expiration:

12 months from the date of shipment when stored properly.

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