Anti-INPP5F Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Gene Id	22876
Protein Name / Synonyms	INPP5F
Concentration (lot specific)	0.79 mg/ml
Recommended Applications	ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

FLJ13081 antibody, KIAA0966 antibody, MGC131851 antibody, MGC59773 antibody, MSTPO47 antibody, SAC2 antibody, hSAC2 antibody, INPP5F antibody, sac domain-containing phosphoinositide 5-phosphatase 2 antibody, Sac domain-containing inositol phosphatase 2 antibody, phosphatidylinositide phosphatase SAC2 antibody, inositol polyphosphate-5-phosphatase F antibody

Specificity

Species Reactivity

Human, Mouse

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

128 kDa

Immunogen

Recombinant protein fragment contain a sequence corresponding to a region within amino acids 593 and 904 of INPP5F

Applications

Applications Tested

ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunofluorescence analysis of methanol-fixed HeLa, using INPP5F(107-10442) antibody at 1:200 dilution.

Immunohistochemical analysis of paraffin-embedded Colon ca, using INPP5F(107-10442) antibody at 1:500 dilution.

Sample (50 ug of whole cell lysate)
A: mouse brain
5% SDS PAGE
107-10442 diluted at 1:500

Sample (30 ug of whole cell lysate)
A: Hela
7.5% SDS PAGE
INPP5F antibody
107-10442 diluted at 1:1000

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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