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Anti-IRAK1 Antibody
Catalog #: 144-12624
IRAK1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P51617 |
| Gene Id | 3654 |
| Gene Symbols | IRAK1 |
| Protein Name / Synonyms | IRAK1, IRAK, pelle, interleukin-1 receptor-associated kinase 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes the interleukin-1 receptor-associated kinase 1, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. This gene is partially responsible for IL1-induced upregulation of the transcription factor NF-kappa B. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Dilutions
WB 1:1000 - 1:3000
IF 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
68kDa/73kDa/74kDa/76kDa
Observed MW
90-100kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 424-633 of human IRAK1 (NP_001020414.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using IRAK1 antibody (144-600844) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Immunofluorescence analysis of C6 cells using IRAK1 antibody (144-600844) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using IRAK1 antibody (144-600844) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using IRAK1 antibody (144-600844) at dilution of 1:100. Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.