Anti-IRAK2 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	O43187
Gene Id	3656
Gene Symbols	IRAK2
Protein Name / Synonyms	IRAK2, IRAK-2, interleukin 1 receptor associated kinase 2
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF
Research Area	Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Background

IRAK2 encodes the interleukin-1 receptor-associated kinase 2, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. IRAK2 is reported to participate in the IL1-induced upregulation of NF-kappaB.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200

Format

Calculated MW

69kDa

Observed MW

75kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human IRAK2 (NP_001561.3).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using IRAK2 antibody (144-06635) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - IRAK2 antibody (144-06635)

Immunofluorescence analysis of A549 cells using IRAK2 antibody (144-06635). Blue: DAPI for nuclear staining.
Immunofluorescence - IRAK2 antibody (144-06635)

Expiration:

12 months from the date of shipment when stored properly.

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