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Anti-IRAK4 Antibody
Catalog #: 144-06208
IRAK4 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q9NWZ3 |
| Gene Id | 51135 |
| Gene Symbols | IRAK4 |
| Protein Name / Synonyms | IRAK4, IPD1, IRAK-4, NY-REN-64, REN64, interleukin-1 receptor-associated kinase 4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a kinase that activates NF-kappaB in both the Toll-like receptor (TLR) and T-cell receptor (TCR) signaling pathways. The protein is essential for most innate immune responses. Mutations in this gene result in IRAK4 deficiency and recurrent invasive pneumococcal disease. Multiple transcript variants encoding different isoforms have been found for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
37kDa/51kDa
Observed MW
55kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human IRAK4 (NP_057207.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using IRAK4 antibody (144-06208) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat testis using IRAK4 antibody (144-06208) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat ovary using IRAK4 antibody (144-06208) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using IRAK4 antibody (144-06208) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using IRAK4 antibody (144-06208) at dilution of 1:100 (20x lens).
Immunohistochemistry of paraffin-embedded mouse brain using IRAK4 antibody (144-06208) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.