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Anti-IRF1 Antibody
Catalog #: 144-07692
IRF1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P10914 |
| Gene Id | 3659 |
| Gene Symbols | IRF1 |
| Protein Name / Synonyms | IRF1, IRF-1, MAR, interferon regulatory factor 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Research Area | Cancer |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
IRF1 encodes interferon regulatory factor 1, a member of the interferon regulatory transcription factor (IRF) family. IRF1 serves as an activator of interferons alpha and beta transcription, and in mouse it has been shown to be required for double-stranded RNA induction of these genes. IRF1 also functions as a transcription activator of genes induced by interferons alpha, beta, and gamma. Further, IRF1 has been shown to play roles in regulating apoptosis and tumor-suppressoion.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IHC 1:50 - 1:100
Format
Calculated MW
36kDa
Observed MW
37kDa
Immunogen
A synthetic peptide of human IRF1
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using IRF1 antibody (144-07692) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse spleen using IRF1 antibody (144-07692) (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using IRF1 antibody (144-07692) (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using IRF1 antibody (144-07692) (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using IRF1 antibody (144-07692) (40x lens).
Expiration:
12 months from the date of shipment when stored properly.