Anti-IRF3 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q14653
Gene Id	3661
Gene Symbols	IRF3
Protein Name / Synonyms	IRF3, IIAE7, interferon regulatory factor 3
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Concentration (lot specific)	0.37 mg/ml
Buffers	PBS with 0.09% sodium azide, 50% glycerol, pH 7.3
Recommended Applications	Western Blotting, IHC, IF
Research Area	Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW

12kDa/16kDa/33kDa/47kDa/49kDa

Observed MW

47kDa

Immunogen

A synthetic peptide of human IRF3

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using IRF3 antibody (144-02172) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Western blot - IRF3 Polyclonal Antibody (144-02172)

Immunofluorescence analysis of HeLa cells using IRF3 antibody (144-02172) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - IRF3 Polyclonal Antibody (144-02172)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products