Anti-IRF3 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q14653
Gene Id	3661
Gene Symbols	IRF3
Protein Name / Synonyms	IRF3, IIAE7, interferon regulatory factor 3
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.01% thimerosal, 50% glycerol, pH 7.3
Recommended Applications	Western Blotting, IHC, ChIP
Research Area	Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
ChIP 1:20 - 1:100

Format

Calculated MW

12kDa/16kDa/33kDa/47kDa/49kDa

Observed MW

56kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human IRF3 (NP_001184051.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using IRF3 antibody (144-11118) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Western blot - [KO Validated] IRF3 Polyclonal Antibody (144-11118)

Western blot analysis of extracts from normal (control) and IRF3 knockout (KO) HeLa cells, using IRF3 antibody (144-11118) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3s.
Western blot - [KO Validated] IRF3 Polyclonal Antibody (144-11118)

Immunohistochemistry of paraffin-embedded human lung cancer using IRF3 antibody (144-11118) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] IRF3 Polyclonal Antibody (144-11118)

Immunohistochemistry of paraffin-embedded human esophageal cancer using IRF3 antibody (144-11118) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] IRF3 Polyclonal Antibody (144-11118)

Chromatin immunoprecipitation analysis extracts of HCT116 cell line, using IRF3 rabbit polyclonal antibody (144-11118) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Expiration:

12 months from the date of shipment when stored properly.

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