Anti-IRF5 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Rat
Host Species	Rabbit
Accession Number	Q13568
Gene Id	3663
Gene Symbols	IRF5
Protein Name / Synonyms	IRF5, SLEB10, interferon regulatory factor 5
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF
Research Area	Inflammation, Autoimmune Disease
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a member of the interferon regulatory factor (IRF) family, a group of transcription factors with diverse roles, including virus-mediated activation of interferon, and modulation of cell growth, differentiation, apoptosis, and immune system activity. Members of the IRF family are characterized by a conserved N-terminal DNA-binding domain containing tryptophan (W) repeats. Alternative promoter use and alternative splicing result in multiple transcript variants, and a 30-nt indel polymorphism (SNP rs60344245) can result in loss of a 10-aa segment.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200

Format

Calculated MW

17kDa/47kDa/54kDa/56kDa/57kDa

Observed MW

57kDa, 60kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human IRF5 (NP_001092097.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using IRF5 antibody (144-01149) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Western blot - IRF5 antibody (144-01149)

Immunofluorescence analysis of HeLa cells using IRF5 antibody (144-01149). Blue: DAPI for nuclear staining.
Immunofluorescence - IRF5 antibody (144-01149)

Expiration:

12 months from the date of shipment when stored properly.

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