Anti-IRF8 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q02556
Gene Id	3394
Gene Symbols	IRF8
Protein Name / Synonyms	IRF8, H-ICSBP, ICSBP, ICSBP1, IMD32A, IMD32B, IRF-8, interferon regulatory factor 8
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF
Research Area	Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Background

Interferon consensus sequence-binding protein (ICSBP) is a transcription factor of the interferon (IFN) regulatory factor (IRF) family. Proteins of this family are composed of a conserved DNA-binding domain in the N-terminal region and a divergent C-terminal region that serves as the regulatory domain. The IRF family proteins bind to the IFN-stimulated response element (ISRE) and regulate expression of genes stimulated by type I IFNs, namely IFN-alpha and IFN-beta. IRF family proteins also control expression of IFN-alpha and IFN-beta-regulated genes that are induced by viral infection.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200

Format

Calculated MW

48kDa

Observed MW

54kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 137-426 of human IRF8 (NP_002154.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of THP-1 cells, using IRF8 antibody (144-60440) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - IRF8 antibody (144-60440)

Immunofluorescence analysis of A549 cells using IRF8 antibody (144-60440).
Immunofluorescence - IRF8 antibody (144-60440)

Expiration:

12 months from the date of shipment when stored properly.

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