Anti-IRS2 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q9Y4H2
Gene Id	8660
Gene Symbols	IRS2
Protein Name / Synonyms	IRS2, IRS-2, insulin receptor substrate 2
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes the insulin receptor substrate 2, a cytoplasmic signaling molecule that mediates effects of insulin, insulin-like growth factor 1, and other cytokines by acting as a molecular adaptor between diverse receptor tyrosine kinases and downstream effectors. The product of this gene is phosphorylated by the insulin receptor tyrosine kinase upon receptor stimulation, as well as by an interleukin 4 receptor-associated kinase in response to IL4 treatment.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:100

Format

Calculated MW

137kDa

Observed MW

170kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 510-650 of human IRS2 (NP_003740.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using IRS2 antibody (144-07945) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Western blot - IRS2 antibody (144-07945)

Immunohistochemistry of paraffin-embedded mouse stomach using IRS2 antibody (144-07945) at dilution of 1:100 (40x lens).
Immunohistochemistry - IRS2 antibody (144-07945)

Immunohistochemistry of paraffin-embedded human esophagus using IRS2 antibody (144-07945) at dilution of 1:100 (40x lens).
Immunohistochemistry - IRS2 antibody (144-07945)

Expiration:

12 months from the date of shipment when stored properly.

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