Anti-ISL2 (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q96A47
Gene Id	64843
Gene Symbols	ISL2
Protein Name / Synonyms	ISL2 (N-term)
Clonality	Polyclonal
Preparation	This ISL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human ISL2.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	IF, Western Blotting
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

ISL2 is a transcriptional factor that defines subclasses of motoneurons that segregate into columns in the spinal cord and select distinct axon pathways (By similarity).

Specificity

Predicted Reactivity

Mouse, Rat

Other Names

ISL2; Insulin gene enhancer protein ISL-2

NCBI Accession #

NP_665804.1

Other Accession #

P50480; Q9CXV0; NP_665804.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

39768

Recommended Dilutions

IF: 1:10-50; WB: 1:1000

Fluorescent image of HUVEC cell stained with ISL2 Antibody (N-term)(Cat# 102-13031).HUVEC cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ISL2 primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°).ISL2 immunoreactivity is localized to Nucleus significantly.

ISL2 Antibody (N-term) (Cat# 102-13031) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the ISL2 antibody detected the ISL2 protein (arrow).

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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