Anti-ITGA2 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P17301
Gene Id	3673
Gene Symbols	ITGA2
Protein Name / Synonyms	ITGA2, BR, CD49B, GPIa, HPA-5, VLA-2, VLAA2, integrin alpha-2
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes the alpha subunit of a transmembrane receptor for collagens and related proteins. The encoded protein forms a heterodimer with a beta subunit and mediates the adhesion of platelets and other cell types to the extracellular matrix. Loss of the encoded protein is associated with bleeding disorder platelet-type 9. Antibodies against this protein are found in several immune disorders, including neonatal alloimmune thrombocytopenia. This gene is located adjacent to a related alpha subunit gene. Alternative splicing results in multiple transcript variants.

Dilutions

WB 1:500 - 1:2000
IHC 1:100 - 1:200
IF 1:50 - 1:200

Format

Calculated MW

129kDa

Observed MW

150kDa

Immunogen

Recombinant protein of human ITGA2

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using ITGA2 antibody (144-07629) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.
Western blot - [KO Validated] ITGA2 Polyclonal Antibody (144-07629)

Western blot analysis of extracts from normal (control) and ITGA2 knockout (KO) HeLa cells, using ITGA2 antibody (144-07629) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - [KO Validated] ITGA2 Polyclonal Antibody (144-07629)

Immunohistochemistry of paraffin-embedded rat brain using ITGA2 Antibody (144-07629) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] ITGA2 Polyclonal Antibody (144-07629)

Immunohistochemistry of paraffin-embedded mouse brain using ITGA2 Antibody (144-07629) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] ITGA2 Polyclonal Antibody (144-07629)

Immunofluorescence analysis of U2OS cells using ITGA2 antibody (144-07629) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - [KO Validated] ITGA2 Polyclonal Antibody (144-07629)

Expiration:

12 months from the date of shipment when stored properly.

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