Anti-ITGA6 (isoform 2 S1064) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P23229
Gene Id	3655
Gene Symbols	ITGA6
Protein Name / Synonyms	ITGA6 (isoform 2 S1064)
Clonality	Polyclonal
Preparation	This ITGA6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1043-1072 amino acids from human ITGA6.
Concentration (lot specific)	0.410 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

The ITGA6 protein product is the integrin alpha chain alpha 6. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, alpha 6 may combine with beta 4 in the integrin referred to as TSP180, or with beta 1 in the integrin VLA-6. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling.

Specificity

Predicted Reactivity

Chicken

Other Names

ITGA6; Integrin alpha-6; CD49 antigen-like family member F; VLA-6; CD_antigen=CD49f; Integrin alpha-6 heavy chain; Integrin alpha-6 light chain

NCBI Accession #

NP_000201.2;NP_001073286.1

Other Accession #

P26007

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

126606

Recommended Dilutions

IF: 1:200; WB: 1:1000; IHC: 1:10-50

	Fluorescent confocal image of HeLa cells stained with ITGA6 (isoform 2 S1064) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with 102-15120 ITGA6 (isoform 2 S1064) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 5 min). Note the highly specific localization of the ITGA6 mainly to the nucleus, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000091409).
	Western blot analysis of anti-ITGA6 Antibody (isoform 2 S1064) (Cat# 102-15120) in 293 cell line lysates (35ug/lane). ITGA6(arrow) was detected using the purified Pab.
	Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with ITGA6 Antibody (isoform 2 S1064) (Cat# 102-15120), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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