Anti-ITGAM Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P11215
Gene Id	3684
Gene Symbols	ITGAM
Protein Name / Synonyms	ITGAM, CD11B, CR3A, MAC-1, MAC1A, MO1A, SLEB6, integrin alpha-M
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.09% sodium azide, 50% glycerol, pH 7.3
Recommended Applications	Western Blotting, IHC
Research Area	Inflammation, Autoimmune Disease
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene.

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW

127kDa

Observed MW

180kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 20-330 of human ITGAM (NP_001139280.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using ITGAM antibody (144-01581) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - ITGAM Polyclonal Antibody (144-01581)

Expiration:

12 months from the date of shipment when stored properly.

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