Anti-ITGB8 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P26012
Gene Id	3696
Gene Symbols	ITGB8
Protein Name / Synonyms	ITGB8, integrin beta-8
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Concentration (lot specific)	1.52 mg/ml
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC
Research Area	Angiogenesis
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene is a member of the integrin beta chain family and encodes a single-pass type I membrane protein with a VWFA domain and four cysteine-rich repeats. This protein noncovalently binds to an alpha subunit to form a heterodimeric integrin complex. In general, integrin complexes mediate cell-cell and cell-extracellular matrix interactions and this complex plays a role in human airway epithelial proliferation. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200

Format

Calculated MW

71kDa/85kDa

Observed MW

100kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 43-320 of human ITGB8 (NP_002205.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using ITGB8 antibody (144-08433) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.
Western blot - ITGB8 antibody (144-08433)

Immunohistochemistry of paraffin-embedded rat brain using ITGB8 antibody (144-08433) at dilution of 1:100 (40x lens).
Immunohistochemistry - ITGB8 antibody (144-08433)

Immunohistochemistry of paraffin-embedded mouse brain using ITGB8 antibody (144-08433) at dilution of 1:100 (40x lens).
Immunohistochemistry - ITGB8 antibody (144-08433)

Expiration:

12 months from the date of shipment when stored properly.

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