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Anti-ITPKB Antibody
Catalog #: 144-03929
ITPKB Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P27987 |
| Gene Id | 3707 |
| Gene Symbols | ITPKB |
| Protein Name / Synonyms | ITPKB, IP3-3KB, IP3K, IP3K-B, IP3KB, PIG37, inositol-trisphosphate 3-kinase B |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Research Area | Akt Signaling |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this protein regulates inositol phosphate metabolism by phosphorylation of second messenger inositol 1,4,5-trisphosphate to Ins(1,3,4,5)P4. The activity of this encoded protein is responsible for regulating the levels of a large number of inositol polyphosphates that are important in cellular signaling. Both calcium/calmodulin and protein phosphorylation mechanisms control its activity.
Dilutions
WB 1:200 - 1:2000
IHC 1:20 - 1:200
IHC 1:20 - 1:200
Format
Calculated MW
67kDa/102kDa
Observed MW
102kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human ITPKB (NP_002212.3).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using ITPKB antibody (144-03929) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Immunohistochemistry of paraffin-embedded human liver injury using ITPKB antibody (144-03929) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using ITPKB antibody (144-03929) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using ITPKB antibody (144-03929) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using ITPKB antibody (144-03929) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.