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Anti-JAK3 Antibody
Catalog #: 144-00748
JAK3 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P52333 |
| Gene Id | 3718 |
| Gene Symbols | JAK3 |
| Protein Name / Synonyms | JAK3, JAK-3, JAK3_HUMAN, JAKL, L-JAK, LJAK, Janus kinase 3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene is a member of the Janus kinase (JAK) family of tyrosine kinases involved in cytokine receptor-mediated intracellular signal transduction. It is predominantly expressed in immune cells and transduces a signal in response to its activation via tyrosine phosphorylation by interleukin receptors. Mutations in this gene are associated with autosomal SCID (severe combined immunodeficiency disease).
Dilutions
WB 1:500 - 1:2000
Format
Calculated MW
68kDa/121kDa/125kDa
Observed MW
125kDa
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 750-850 of human JAK3 (NP_000206.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of U-87MG cells, using JAK3 antibody (144-00748) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Expiration:
12 months from the date of shipment when stored properly.