Anti-JUP Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	P14923
Gene Id	3728
Gene Symbols	JUP
Protein Name / Synonyms	JUP, ARVD12, CTNNG, DP3, DPIII, PDGB, PKGB, junction plakoglobin
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF, IP
Research Area	Angiogenesis, Cardiovascular Disease
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a major cytoplasmic protein which is the only known constituent common to submembranous plaques of both desmosomes and intermediate junctions. This protein forms distinct complexes with cadherins and desmosomal cadherins and is a member of the catenin family since it contains a distinct repeating amino acid motif called the armadillo repeat. Mutation in this gene has been associated with Naxos disease. Alternative splicing occurs in this gene; however, not all transcripts have been fully described.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200
IP 1:50 - 1:100

Format

Calculated MW

81kDa

Observed MW

82kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human JUP (NP_002221.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using JUP antibody (144-00963) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Western blot - JUP Polyclonal Antibody (144-00963)

Immunofluorescence analysis of HeLa cells using JUP antibody (144-00963). Blue: DAPI for nuclear staining.
Immunofluorescence - JUP Polyclonal Antibody (144-00963)

Immunoprecipitation analysis of 200ug extracts of MCF-7 cells, using 3 ug JUP antibody (144-00963). Western blot was performed from the immunoprecipitate using JUP antibody (144-00963) at a dilition of 1:1000.
Immunoprecipitation - JUP Polyclonal Antibody (144-00963)

Expiration:

12 months from the date of shipment when stored properly.

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