Anti-KAT5 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q92993
Gene Id	10524
Gene Symbols	KAT5
Protein Name / Synonyms	KAT5, ESA1, HTATIP, HTATIP1, PLIP, TIP, TIP60, ZC2HC5, cPLA2, lysine acetyltransferase 5
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF
Research Area	Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene belongs to the MYST family of histone acetyl transferases (HATs) and was originally isolated as an HIV-1 TAT-interactive protein. HATs play important roles in regulating chromatin remodeling, transcription and other nuclear processes by acetylating histone and nonhistone proteins. This protein is a histone acetylase that has a role in DNA repair and apoptosis and is thought to play an important role in signal transduction. Alternative splicing of this gene results in multiple transcript variants.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200

Format

Calculated MW

53kDa/56kDa/58kDa/61kDa

Observed MW

62kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human KAT5 (NP_006379.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using KAT5 antibody (144-01678) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - KAT5 Polyclonal Antibody (144-01678)

Immunofluorescence analysis of U2OS cells using KAT5 antibody (144-01678) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - KAT5 Polyclonal Antibody (144-01678)

Expiration:

12 months from the date of shipment when stored properly.

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