Anti-KAT7 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	O95251
Gene Id	11143
Gene Symbols	KAT7
Protein Name / Synonyms	KAT7, HBO1, HBOA, MYST2, ZC2HC7, lysine acetyltransferase 7
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene is part of the multimeric HBO1 complex, which possesses histone H4-specific acetyltransferase activity. This activity is required for functional replication origins and is involved in transcriptional activation of some genes. In both cases, the acetylation of histone H4 helps unfold chromatin so that the DNA can be accessed and replicated or transcribed.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200

Format

Calculated MW

51kDa/55kDa/58kDa/66kDa/70kDa

Observed MW

63kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-320 of human KAT7 (NP_008998.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using KAT7 antibody (144-05823) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - KAT7 Polyclonal Antibody (144-05823)

Immunofluorescence analysis of HeLa cells using KAT7 antibody (144-05823) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence - KAT7 Polyclonal Antibody (144-05823)

Expiration:

12 months from the date of shipment when stored properly.

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