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Anti-KCNJ4 Antibody

Catalog #: 144-60548

KCNJ4 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Accession Number
P48050
Gene Id
3761
Gene Symbols
KCNJ4
Protein Name / Synonyms
KCNJ4, HIR, HIRK2, HRK1, IRK-3, IRK3, Kir2.3, inward rectifier potassium channel 4
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting, IHC
Shipping TypeBlue ice
Storage-20°C

Description

Background

Several different potassium channels are known to be involved with electrical signaling in the nervous system. One class is activated by depolarization whereas a second class is not. The latter are referred to as inwardly rectifying K+ channels, and they have a greater tendency to allow potassium to flow into the cell rather than out of it. This asymmetry in potassium ion conductance plays a key role in the excitability of muscle cells and neurons. The protein encoded by this gene is an integral membrane protein and member of the inward rectifier potassium channel family. The encoded protein has a small unitary conductance compared to other members of this protein family. Two transcript variants encoding the same protein have been found for this gene.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200

Format

Calculated MW
49kDa
Observed MW
47kDa

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 50-150 of human KCNJ4 (NP_004972.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using KCNJ4 antibody (144-014010) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 30s.
Western blot - KCNJ4 antibody (144-014010)

Immunohistochemistry of paraffin-embedded rat brain using KCNJ4 antibody (144-014010) at dilution of 1:100 (40x lens).
Immunohistochemistry - KCNJ4 antibody (144-014010)

Expiration:
12 months from the date of shipment when stored properly.