RayBiotech

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ISO 13485:2016 cGMP

Anti-KCTD15 Antibody

Catalog #: 144-08256

KCTD15 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Accession Number
Q96SI1
Gene Id
79047
Gene Symbols
KCTD15
Protein Name / Synonyms
KCTD15, BTB/POZ domain-containing protein KCTD15
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting, IHC, IF
Research AreaCardiovascular Disease
Shipping TypeBlue ice
Storage-20°C

Description

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100

Format

Calculated MW
26kDa/31kDa
Observed MW
32kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-234 of human KCTD15 (NP_076981.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using KCTD15 antibody (144-08256) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - KCTD15 antibody (144-08256)

Immunohistochemistry of paraffin-embedded mouse lung using KCTD15 antibody (144-08256) at dilution of 1:100 (40x lens).
Immunohistochemistry - KCTD15 antibody (144-08256)

Immunohistochemistry of paraffin-embedded rat lung using KCTD15 antibody (144-08256) at dilution of 1:100 (40x lens).
Immunohistochemistry - KCTD15 antibody (144-08256)

Immunohistochemistry of paraffin-embedded human esophagus using KCTD15 antibody (144-08256) at dilution of 1:100 (40x lens).
Immunohistochemistry - KCTD15 antibody (144-08256)

Immunofluorescence analysis of U2OS cells using KCTD15 antibody (144-08256) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - KCTD15 antibody (144-08256)

Expiration:
12 months from the date of shipment when stored properly.