Anti-KDM1A Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	O60341
Gene Id	23028
Gene Symbols	KDM1A
Protein Name / Synonyms	KDM1A, AOF2, BHC110, CPRF, KDM1, LSD1, lysine demethylase 1A
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF, IP
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. Alternative splicing results in multiple transcript variants.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100
IP 1:50 - 1:100

Format

Calculated MW

92kDa/95kDa

Observed MW

96-117kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 130-380 of human KDM1A (NP_055828.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using KDM1A antibody (144-01156) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Western blot - [KO Validated] KDM1A Polyclonal Antibody (144-01156)

Western blot analysis of extracts from normal (control) and KDM1A knockout (KO) HeLa cells, using KDM1A antibody (144-01156) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - [KO Validated] KDM1A Polyclonal Antibody (144-01156)

Immunofluorescence analysis of A549 cells using KDM1A antibody (144-01156).
Immunofluorescence - [KO Validated] KDM1A Polyclonal Antibody (144-01156)

Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug KDM1A antibody (144-01156). Western blot was performed from the immunoprecipitate using KDM1A antibody (144-01156) at a dilition of 1:500.
Immunoprecipitation - [KO Validated] KDM1A Polyclonal Antibody (144-01156)

Expiration:

12 months from the date of shipment when stored properly.

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