KDM4A Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | O75164 |
| Gene Id | 9682 |
| Gene Symbols | KDM4A |
| Protein Name / Synonyms | KDM4A, JHDM3A, JMJD2, JMJD2A, TDRD14A, lysine demethylase 4A |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IP |
| Research Area | Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene is a member of the Jumonji domain 2 (JMJD2) family and encodes a protein containing a JmjN domain, a JmjC domain, a JD2H domain, two TUDOR domains, and two PHD-type zinc fingers. This nuclear protein functions as a trimethylation-specific demethylase, converting specific trimethylated histone residues to the dimethylated form, and as a transcriptional repressor.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IP 1:50 - 1:100
IHC 1:50 - 1:200
IP 1:50 - 1:100
Format
Calculated MW
54kDa/120kDa
Observed MW
150kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 585-815 of human KDM4A (NP_055478.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using KDM4A antibody (144-07953) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse lung using KDM4A antibody (144-07953) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using KDM4A antibody (144-07953) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using KDM4A antibody (144-07953) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.