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Anti-KDR Antibody
Catalog #: 144-11127
KDR Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P35968 |
| Gene Id | 3791 |
| Gene Symbols | KDR |
| Protein Name / Synonyms | KDR, CD309, FLK1, VEGFR, VEGFR2, kinase insert domain receptor |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Research Area | Angiogenesis, Cancer, Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
75kDa/79kDa/151kDa
Observed MW
265kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1147-1356 of human KDR (NP_002244.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of mouse skeletal muscle, using KDR antibody (144-11127) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat testis using KDR antibody (144-11127) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using KDR antibody (144-11127) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using KDR antibody (144-11127) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.