Anti-KHK Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P50053
Gene Id	3795
Gene Symbols	KHK
Protein Name / Synonyms	KHK, ketohexokinase
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes ketohexokinase that catalyzes conversion of fructose to fructose-1-phosphate. The product of this gene is the first enzyme with a specialized pathway that catabolizes dietary fructose. Alternatively spliced transcript variants encoding different isoforms have been identified.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200

Format

Calculated MW

32kDa

Observed MW

33kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-298 of human KHK (NP_000212.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using KHK antibody (144-07440) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - KHK Polyclonal Antibody (144-07440)

Immunohistochemistry of paraffin-embedded human kidney using KHK antibody (144-07440) at dilution of 1:100 (40x lens).
Immunohistochemistry - KHK Polyclonal Antibody (144-07440)

Expiration:

12 months from the date of shipment when stored properly.

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