RayBiotech

3607 Parkway Ln Ste 200 Norcross, Georgia 30092

www.RayBiotech.com | info@raybiotech.com

770-729-2992 | +1-888-494-8555

ISO 13485:2016 cGMP

Anti-Ki-67 (1010-1033aa) Antibody

Catalog #: 130-11077

Ki-67 (1010-1033aa) Antibody

Product Description

Specifications

Size20 µg, 100 µg, 200 µg, 500 µg, 1 mg
Estimated Lead Time1-2 business days
SpeciesHuman
Host SpeciesRabbit
ClonalityPolyclonal
PreparationThis antibody was produced from antiserum of rabbits immunized with a recombinant protein immunogen. The IgG fraction was purified by Protein A affinity chromatography.
Recommended ApplicationsELISA (recommended work dilution = 1:5, 000)
Reconstitution
Product is supplied as a powder obtained from lyophilization of purified antibody in glycine solution. Reconstitute the antibody with sterile water to a final concentration of 1 mg/ml.
Shipping TypeAmbient temperature
Storage-20°C

Description

Description

This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X.

Indirect Fluorescent Antibody (IFA) Analysis

1130-11077 (1:100): Rabbit Anti-Ki-67 (1010-1033aa)
Alexa Fluor 488 goat anti-rabbit (1:200)
Hela cells
Subcellular Location: nucleus, nucleolus


1130-11077 (1:100): Rabbit Anti-Ki-67 (1010-1033aa)
CY3 goat anti-rabbit (1:200)
Hela cells
Subcellular Location: nucleus, nucleolus

Immunohistochemistry (IHC) Analysis

IHC shows positive staining in colon cancer tumor tissue. Antibody Rabbit Anti-Human Ki-67 (1010-1033aa) was used at a dilution of 1/200 followed by mouse and rabbit IgG (ready to use) polymerization using HRP polymer, counterstained with hematoxylin. Heat-mediated antigen retrieval was performed using Tris/EDTA buffer pH9.0 before starting IHC staining (40X).

Storage/Stability

Store at -20°C. If intended for use within a month, may keep at 4°C. Minimize freeze-thaw cycles.

Expiration

12 months from the date of shipment when stored properly.