Anti-KIF11 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P52732
Gene Id	3832
Gene Symbols	KIF11
Protein Name / Synonyms	KIF11, EG5, HKSP, KNSL1, MCLMR, TRIP5, kinesin family member 11
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a motor protein that belongs to the kinesin-like protein family. Members of this protein family are known to be involved in various kinds of spindle dynamics. The function of this gene product includes chromosome positioning, centrosome separation and establishing a bipolar spindle during cell mitosis.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200

Format

Calculated MW

119kDa

Observed MW

130kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 797-1056 of human KIF11 (NP_004514.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using KIF11 antibody (144-07907) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Western blot - KIF11 antibody (144-07907)

Immunofluorescence analysis of U2OS cells using KIF11 antibody (144-07907) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - KIF11 antibody (144-07907)

Expiration:

12 months from the date of shipment when stored properly.

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