3607 Parkway Ln Ste 200 Norcross, Georgia 30092
www.RayBiotech.com | info@raybiotech.com
770-729-2992 | +1-888-494-8555
ISO 13485:2016 cGMP
Anti-KIF14 Antibody
Catalog #: 144-10275
KIF14 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q15058 |
| Gene Id | 9928 |
| Gene Symbols | KIF14 |
| Protein Name / Synonyms | KIF14, MKS12, kinesin family member 14 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a member of the kinesin-3 superfamily of microtubule motor proteins. These proteins are involved in numerous processes including vesicle transport, chromosome segregation, mitotic spindle formation, and cytokinesis. In human HeLa-S3 and 293T cells, this protein is localized to the cytoplasm during interphase, to the spindle poles and spindle microtubules during mitosis, and to the midbody during cytokinesis. An internal motor domain displays microtubule-dependent ATPase activity, consistent with its function as a microtubule motor protein. Knockdown of this gene results in failed cytokinesis with endoreplication, which results in multinucleated cells. This gene has been identified as a likely oncogene in breast, lung and ovarian cancers, as well as retinoblastomas and gliomas. Alternative splicing results in multiple transcript variants.
Dilutions
WB 1:1000 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
186kDa
Observed MW
190kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1399-1648 of human KIF14 (NP_055690.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using KIF14 antibody (144-10275) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.
Immunohistochemistry of paraffin-embedded rat ovary using KIF14 antibody (144-10275) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using KIF14 antibody (144-10275) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using KIF14 antibody (144-10275) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human appendix using KIF14 antibody (144-10275) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human mammary cancer using KIF14 antibody (144-10275) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using KIF14 antibody (144-10275) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.