Anti-KIF4A Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	O95239
Gene Id	24137
Gene Symbols	KIF4A
Protein Name / Synonyms	KIF4A, KIF4, KIF4G1, MRX100, kinesin family member 4A
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a member of the kinesin 4 subfamily of kinesin related proteins. The encoded protein is an ATP dependent microtubule-based motor protein that is involved in the intracellular transport of membranous organelles. This protein also associates with condensed chromosome arms and may be involved in maintaining chromosome integrity during mitosis. This protein may also be involved in the organization of the central spindle prior to cytokinesis. A pseudogene of this gene is found on chromosome X.

Dilutions

WB 1:1000 - 1:3000
IHC 1:50 - 1:200
IF 1:100 - 1:200

Format

Calculated MW

128kDa/139kDa

Observed MW

140kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 870-1080 of human KIF4A (NP_036442.3).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using KIF4A antibody (144-10193) at 1:7000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Western blot - KIF4A Polyclonal Antibody (144-10193)

Immunofluorescence analysis of HeLa cells using KIF4A antibody (144-10193) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence - KIF4A Polyclonal Antibody (144-10193)

Expiration:

12 months from the date of shipment when stored properly.

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