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Anti-KIF5A Antibody
Catalog #: 144-03303
KIF5A Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q12840 |
| Gene Id | 3798 |
| Gene Symbols | KIF5A |
| Protein Name / Synonyms | KIF5A, D12S1889, MY050, NEIMY, NKHC, SPG10, kinesin family member 5A |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a member of the kinesin family of proteins. Members of this family are part of a multisubunit complex that functions as a microtubule motor in intracellular organelle transport. Mutations in this gene cause autosomal dominant spastic paraplegia 10.
Dilutions
WB 1:1000 - 1:3000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
117kDa
Observed MW
125kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 933-1032 of human KIF5A (NP_004975.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using KIF5A antibody (144-03303) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded mouse brain using KIF5A antibody (144-03303) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.