Anti-KLF4 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	O43474
Gene Id	9314
Gene Symbols	KLF4
Protein Name / Synonyms	KLF4, EZF, GKLF, Kruppel like factor 4
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Research Area	Angiogenesis, Cancer, Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a protein that belongs to the Kruppel family of transcription factors. The encoded zinc finger protein is required for normal development of the barrier function of skin. The encoded protein is thought to control the G1-to-S transition of the cell cycle following DNA damage by mediating the tumor suppressor gene p53. Mice lacking this gene have a normal appearance but lose weight rapidly, and die shortly after birth due to fluid evaporation resulting from compromised epidermal barrier function. Alternative splicing results in multiple transcript variants encoding different isoforms.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW

6kDa/12kDa/45kDa/51kDa/54kDa

Observed MW

50kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 180-479 of human KLF4 (NP_004226.3).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using KLF4 antibody (144-06640) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Western blot - KLF4 antibody (144-06640)

Immunohistochemistry of paraffin-embedded human stomach cancer using KLF4 antibody (144-06640) at dilution of 1:200 (40x lens).
Immunohistochemistry - KLF4 antibody (144-06640)

Immunofluorescence analysis of U2OS cells using KLF4 antibody (144-06640) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - KLF4 antibody (144-06640)

Expiration:

12 months from the date of shipment when stored properly.