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Anti-KLF9 (N-term) Antibody

Catalog #: 102-12908

Rabbit Anti-Human KLF9 (N-term) Antibody, 400 ul

Product Description

Specifications

Size400 µL
Estimated Lead Time1-2 weeks
SpeciesHuman
Host SpeciesRabbit
Accession Number
Q13886
Gene Id
687
Gene Symbols
KLF9
Protein Name / Synonyms
KLF9 (N-term)
ClonalityPolyclonal
PreparationThis KLF9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 29-57 amino acids from the N-terminal region of human KLF9.
Concentration (lot specific)
0.390 mg/ml
Recommended ApplicationsWestern Blotting, IF
Shipping TypeBlue ice
Storage-20°C

Description

Introduction

The protein encoded by this gene is a transcription factor that binds to GC box elements located in the promoter. Binding of the encoded protein to a single GC box inhibits mRNA expression while binding to tandemly repeated GC box elements activates transcription.

Specificity

Predicted Reactivity
Mouse, Rat, Pig
Other Names
KLF9; BTEB; BTEB1; Krueppel-like factor 9; GC-box-binding protein 1; Basic transcription element-binding protein 1; Transcription factor BTEB1
NCBI Accession #
NP_001197.1
Other Accession #
Q01713; P79288; O35739; NP_001197.1

Format

Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
27235
Recommended Dilutions
WB: 1:1000; IF: 1:10-50
KLF9 Antibody (N-term) (Cat# 102-12908) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the KLF9 antibody detected the KLF9 protein (arrow).
Fluorescent confocal image of U251 cell stained with KLF9 Antibody (N-term)(Cat# 102-12908).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with KLF9 primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). KLF9 immunoreactivity is localized to vesicles and Nucleus significantly.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.