Anti-KPNA1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	P52294
Gene Id	3836
Gene Symbols	KPNA1
Protein Name / Synonyms	KPNA1, IPOA5, NPI-1, RCH2, SRP1, importin subunit alpha-5
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC), which consists of 60-100 proteins. Small molecules (up to 70 kD) can pass through the nuclear pore by nonselective diffusion while larger molecules are transported by an active process. The protein encoded by this gene belongs to the importin alpha family, and is involved in nuclear protein import. This protein interacts with the recombination activating gene 1 (RAG1) protein and is a putative substrate of the RAG1 ubiquitin ligase. Alternative splicing results in multiple transcript variants.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW

60kDa

Observed MW

66kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human KPNA1 (NP_002255.3).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using KPNA1 antibody (144-60415) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - KPNA1 antibody (144-60415)

Immunofluorescence analysis of U2OS cells using KPNA1 antibody (144-60415). Blue: DAPI for nuclear staining.
Immunofluorescence - KPNA1 antibody (144-60415)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products