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Anti-KRT18 Antibody
Catalog #: 144-60052
KRT18 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P05783 |
| Gene Id | 3875 |
| Gene Symbols | KRT18 |
| Protein Name / Synonyms | KRT18, CK-18, CYK18, K18, keratin 18 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.09% Sodium azide, 50% glycerol, pH 7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Research Area | Cancer |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100
IHC 1:50 - 1:100
IF 1:50 - 1:100
Format
Calculated MW
48kDa
Observed MW
48kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 127-430 of human KRT18 (NP_000215.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using KRT18 antibody (144-60052) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human liver cancer using KRT18 antibody (144-60052) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast using KRT18 antibody (144-60052) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using KRT18 antibody (144-60052) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human placenta using KRT18 antibody (144-60052) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human small intestine using KRT18 antibody (144-60052) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using KRT18 antibody (144-60052) at dilution of 1:100. Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.