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Anti-Ku70 + Ku80 Antibody
Catalog #: 188-10092
Mouse anti-Human/Primates Ku70 + Ku80 Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Specificity | Other species not known |
| Host Species | Mouse |
| Accession Number | P12956 |
| Gene Id | 2547 |
| Gene Symbols | XRCC6,G22P1 |
| Protein Name / Synonyms | X-ray repair cross-complementing protein 6 (EC 3.6.4.-) (EC 4.2.99.-) (5'-deoxyribose-5-phosphate lyase Ku70) (5'-dRP lyase Ku70) (70 kDa subunit of Ku antigen) (ATP-dependent DNA helicase 2 subunit 1) (ATP-dependent DNA helicase II 70 kDa subunit) (CTC box-binding factor 75 kDa subunit) (CTC75) (CTCBF) (DNA repair protein XRCC6) (Lupus Ku autoantigen protein p70) (Ku70) (Thyroid-lupus autoantigen) (TLAA) (X-ray repair complementing defective repair in Chinese hamster cells 6) |
| Immunogen | Nuclear extract of human HL-60 cells was used as the immunogen for this Ku70 + Ku80 antibody. |
| Isotype | Kappa, IgG1 |
| Purity | Protein G purified monoclonal antibody |
| Concentration (lot specific) | 0.2 mg/ml in 1X PBS (pH 7.4) with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide |
| Recommended Applications | Flow cytometry: 0.5-1ug/10e6 cells IF: 0.5-1.0ug/ml Immunocytochemistry (Acetone-fixed cells): 0.25-0.5ug/ml for 30 minutes at RT |
| Domain / Region / Terminus | Nuclear |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Description
Description
This antibody recognizes a dimer of two proteins of 70kDa (Ku70) and ~80kDa (Ku80), identified as two subunits of Ku. Antbody KU729 recognizes a conformational epitope of the Ku70 + Ku80 dimer, which is destroyed during Western blotting. The Ku70 + Ku80 dimer is important for function of a 460kDa DNA-dependent protein kinase. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.
Storage
Store the Ku70 + Ku80 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Expiration:
12 months from the date of shipment when stored properly.