Anti-Ku80 (internal) Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Gene Id	7520
Protein Name / Synonyms	Ku80, Internal
Concentration (lot specific)	1.01 mg/ml
Recommended Applications	ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Immunoprecipitation, Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

FLJ39089 antibody, KARP-1 antibody, KARP1 antibody, KU80 antibody, KUB2 antibody, Ku86 antibody, NFIV antibody, XRCC5 antibody, ATP-dependent DNA helicase II 80 kDa subunit antibody, CTC85 antibody, TLAA antibody, Ku86 autoantigen related protein 1 antibody, nuclear factor IV antibody, 86 kDa subunit of Ku antigen antibody, thyroid-lupus autoantigen antibody, Ku autoantigen, 80kDa antibody, ATP-dependent DNA helicase 2 subunit 2 antibody, DNA repair protein XRCC5 antibody, X-ray repair cross-complementing protein 5 antibody, CTC box-binding factor 85 kDa subunit antibody, CTCBF antibody, lupus Ku autoantigen protein p86 antibody, X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining) antibody

Specificity

Species Reactivity

Human

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

83 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 315 and 593 of Ku80 (Uniprot ID# P13010)

Applications

Applications Tested

ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Immunoprecipitation,Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000, IP: 1:100-1:500. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Ku80(XRCC5) antibody immunoprecipitates Ku80 protein in IP experiments. IP Sample: 1000 µg HeLa whole cell lysate/extract A. 40 µg HeLa whole cell lysate/extract B. Control with 2.5 µg of preimmune rabbit IgG C. Immunoprecipitation of Ku80 protein by 2.5 µg of Ku80 antibody (107-10881) 7.5% SDS-PAGE The immunoprecipitated Ku80 protein was detected by Ku80 antibody (107-10881) diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.

Ku80 antibody [N3C2], Internal detects XRCC5 protein by Western blot analysis.
A. 30 µg 293T whole cell lysate/extract
B. 30 µg A431 whole cell lysate/extract
C. 30 µg HeLa whole cell lysate/extract
D. 30 µg HepG2 whole cell lysate/extract
7.5 % SDS-PAGE
Ku80 antibody [N3C2], Internal (107-10881) dilution: 1:1000

Immunofluorescence analysis of methanol-fixed HeLa, using Ku80 (XRCC5)(107-10881) antibody at 1:200 dilution.

Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using Ku80 (XRCC5)(107-10881) antibody at 1:100 dilution.

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

Featured Products