RayBiotech

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ISO 13485:2016 cGMP

Anti-L3MBTL3 Antibody

Catalog #: 144-07289

L3MBTL3 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse
Host SpeciesRabbit
Accession Number
Q96JM7
Gene Id
84456
Gene Symbols
L3MBTL3
Protein Name / Synonyms
L3MBTL3, MBT-1, MBT1, l(3)mbt-like 3 (Drosophila)
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting, IF, IP
Shipping TypeBlue ice
Storage-20°C

Description

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:100
IP 1:50 - 1:200

Format

Calculated MW
85kDa/88kDa
Observed MW
110kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human L3MBTL3 (NP_001007103.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using L3MBTL3 antibody (144-07289) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 30s.
Western blot - L3MBTL3 antibody (144-07289)

Immunofluorescence analysis of MCF-7 cells using L3MBTL3 antibody (144-07289). Blue: DAPI for nuclear staining.
Immunofluorescence - L3MBTL3 antibody (144-07289)

Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug L3MBTL3 antibody (144-07289). Western blot was performed from the immunoprecipitate using L3MBTL3 antibody (144-07289) at a dilition of 1:1000.
Immunoprecipitation - 1ug L3MBTL3 antibody (144-07289)

Expiration:
12 months from the date of shipment when stored properly.