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Anti-Lambda Light Chain Antibody
Catalog #: 188-11035
Mouse anti-Human Lambda Light Chain Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Specificity | Other species not known |
| Host Species | Mouse |
| Accession Number | P01701|P0CG04 |
| Gene Id | 3535 |
| Gene Symbols | IGLV1-51,IGLC1 |
| Protein Name / Synonyms | Immunoglobulin lambda variable 1-51 (Ig lambda chain V-I region BL2) (Ig lambda chain V-I region EPS) (Ig lambda chain V-I region NEW) (Ig lambda chain V-I region NIG-64), Immunoglobulin lambda constant 1 (Ig lambda chain C region MGC) (Ig lambda-1 chain C region) |
| Immunogen | IgG purified from human serum was used as the immunogen for this Lambda Light Chain antibody. |
| Isotype | Kappa, IgG1 |
| Purity | Protein G affinity chromatography |
| Concentration (lot specific) | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide |
| Recommended Applications | Flow Cytometry: 0.5-1ug/million cells in 0.1ml Immunofluorescence: 1-2ug/ml Western blot: 0.5-1ug/ml Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 minutes at RT |
| Domain / Region / Terminus | Cell Surface, Cytoplasmic and Secreted |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Description
Description
Antibodies are produced by B lymphocytes, each expressing only one class of light chain. Once set, light chain class remains fixed for the life of the B lymphocyte. In a healthy individual, the total kappa to lambda ratio is roughly 3:1 in serum (measuring intact whole antibodies) or 1:1.5 if measuring free light chains, with a highly divergent ratio indicative of neoplasm.
Individual B-cells in lymphoid tissue possess either kappa or lambda light chains, but never both together. Specific rearrangement of lambda light chain of immunoglobulins can lead to loss of some protein coding genes, which does not seem to be functionally relevant (while functionally relevant miR-650 can be overexpressed). Using immunohistochemistry, it is possible to determine the relative abundance of B-cells expressing kappa and lambda light chains. If the lymph node or similar tissue is reactive, or otherwise benign, it should possess a mixture of kappa positive and lambda positive cells. If, however, one type of light chain is significantly more common than the other, the cells are likely all derived from a small clonal population, which may indicate a malignant condition, such as B-cell lymphoma. [Wiki]
Storage
Store the Lambda Light Chain antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Expiration:
12 months from the date of shipment when stored properly.